Ultralink Biosupport Pierce

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UltraLink™ Biosupport

    https://www.thermofisher.com/order/catalog/product/53110
    UltraLink Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilizing antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume.

INSTRUCTIONS UltraLink Biosupport Medium

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkBioSupport.pdf
    Pierce UltraLink® Biosupport Medium is a preactivated, ready-to-use bead designed for use in affinity chromatography. These beads are composed of a bis -acrylamide/azlactone copolymer that is slightly hydrophobic in its active form and highly

Pierce™ Anti-DYKDDDDK Affinity Resin

    https://www.thermofisher.com/order/catalog/product/A36804
    UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity. The porosity, rigidity, and durability of the resin make it suitable for medium-pressure, fast-flow techniques involving large sample volumes.

INSTRUCTIONS UltraLink Biosupport

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011317_UltraLnk_BioSup_UG.pdf
    The Thermo Scientific UltraLink Biosupport is a pre-activated porous resin for use in affinity chromatography. These beads are composed of a bisacrylamide/azlactone copolymer that is slightly hydrophobic in its active form and highly crosslinked.

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-neutravidin-resins/11829410
    NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

0514.5 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011270_UltraLnk_Hydrazide_Resin_UG.pdf
    UltraLink Biosupport is a rigid, highly crosslinked, copolymeric and porous resin with high coupling capacity.2-8 The charge-free support has minimal nonspecific interactions with most sample types. The porosity, rigidity and durability of UltraLink Biosupport make it suitable for medium-pressure, fast-flow techniques involving large sample ...

Protein Purification Support—Getting Started Thermo ...

    https://www.thermofisher.com/us/en/home/technical-resources/technical-reference-library/protein-purification-isolation-support-center/protein-purification-support/protein-purification-support-getting-started.html
    AminoLink™ Plus Support UltraLink™ Biosupport. Pierce™ NHS-Activated Agarose Pierce™ CDI Support. Crosslinked beaded agarose Crosslinked beaded agarose Polyacrylamide/Azlactone Agarose. Agarose. Amine-reactive (Lysine, N-terminal) SulfoLink™ Support UltraLink™ Iodoacetyl Support.

0518.8 Number Description - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011271_Pierce_Streptavidin_UltraLnk_Resin_UG.pdf
    Number Description . 53113 Pierce Streptavidin UltraLink Resin, 2mL settled resin (4mL total volume) ... more rigid support is required if pressures are greater than 25psi UltraLink Biosupport is, therefore, useful for medium- ... Use ~3mg of biotinylated protein/mL of settled Pierce Streptavidin UltraLink …

Protein A/G UltraLink Resin - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011265_Protein_AG_UltraLnk_Resin_UG.pdf
    The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to investigate and purify immunoglobulins. The Thermo Scientific Protein A/G UltraLink Resins use an azlactone-activated support that is hydrophilic, charge-free, high

UltraLink™ Iodoacetyl Resin

    https://www.thermofisher.com/order/catalog/product/53155
    Thermo Scientific UltraLink Iodoacetyl Resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblization of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.Features of UltraLink Iodoacetyl Resin: High

UltraLink™ Biosupport - Thermo Fisher Scientific

    https://www.thermofisher.com/order/catalog/product/53110
    Thermo Scientific UltraLink Biosupport is a durable, azlactone-activated, beaded-polyacrylamide resin to covalently immobilize proteins and other primary amine biomolecules for preparation of affinity purification columns.Features of UltraLink Biosupport: High coupling efficiency and capacityimmobil

INSTRUCTIONS UltraLink Biosupport Medium

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkBioSupport.pdf
    Pierce UltraLink® Biosupport Medium is a preactivated, ready-to-use bead designed for use in affinity chromatography. These beads are composed of a bis-acrylamide/azlactone copolymer that is slightly hydrophobic in its active form and highly cross-linked. Because the azlactone functionality is copolymerized with the matrix material, the ...

INSTRUCTIONS UltraLink Biosupport

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011317_UltraLnk_BioSup_UG.pdf
    The Thermo Scientific UltraLink Biosupport is a pre-activated porous resin for use in affinity chromatography. These beads are composed of a bisacrylamide/azlactone copolymer that is slightly hydrophobic in its active form and highly crosslinked.

Protein A/G UltraLink Resin - Thermo Fisher Scientific

    https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011265_Protein_AG_UltraLnk_Resin_UG.pdf
    The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to investigate and purify immunoglobulins. The Thermo Scientific Protein A/G UltraLink Resins use an azlactone-activated support that is hydrophilic, charge-free, high

Pierce™ Anti-DYKDDDDK Affinity Resin

    https://www.thermofisher.com/order/catalog/product/A36804
    The amino acid sequence DYKDDDDK, commonly known as "FLAG", is recognized by a high-affinity rat monoclonal antibody (clone L5) that is covalently attached to UltraLink Biosupport. UltraLink Biosupport is a rigid, hydrophilic, highly crosslinked, copolymeric and porous resin with high coupling capacity.

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-neutravidin-resins/11829410
    NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Thermo Scientific Pierce Streptavidin Resins :Life ...

    https://www.fishersci.com/shop/products/pierce-streptavidin-resins/p-4531324
    Thermo Scientific™ Pierce Streptavidin Resins comprise a wide selection of high-quality affinity beads and columns for nearly any kind of biotin-based purification procedure. ... The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in ...

Thermo Scientific Pierce NeutrAvidin Resins UltraLink ...

    https://www.fishersci.com/shop/products/pierce-neutravidin-resins/pi53150
    Acrylamide and agarose beads and columns of specially-modified, pH-stabilized avidin; the low-cost alternative to streptavidin biotin-binding supports.

INSTRUCTIONS UltraLink Iodoacetyl Gel

    http://wolfson.huji.ac.il/purification/PDF/affinity/PIERCE_UltralinkIodoAcetyl.pdf
    UltraLink® Iodoacetyl Gel is prepared on UltraLink® Biosupport Medium.2-8 The porosity, rigidity and durability of this support are important considerations when working with large volumes of samples requiring fast-flow techniques and large-scale bulk applications.

Thermo Scientific™ Pierce™ Streptavidin Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-streptavidin-resins/10259973
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.Description: Streptavidin UltraLink Plus Resin

Thermo Scientific™ Pierce™ Streptavidin Resins Fisher ...

    https://www.fishersci.ca/shop/products/pierce-streptavidin-resins/pi53116
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Thermo Scientific™ Pierce™ Streptavidin Resins UltraLink ...

    https://www.fishersci.co.uk/shop/products/pierce-streptavidin-resins/10259973
    The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI. The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11.

Biosupport, UltraLink™ VWR

    https://uk.vwr.com/store/product/18535804/biosupport-ultralinktm
    UltraLink™ Biosupport is an alternative to CNBr- and NHS-activated agarose resins for immobilising antibodies and other proteins to prepare affinity columns. The beaded resin is a hydrophilic copolymer of polyacrylamide and azlactone having a rigid polymeric structure with high surface area and pore volume.

Thermo Scientific™ Pierce™ NeutrAvidin™ Resins Fisher ...

    https://www.fishersci.ca/shop/products/pierce-neutravidin-resins/pi29202
    Thermo Scientific™ Pierce™ NeutrAvidin™ Resins ... NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at …

Cyclin A- and Cyclin E-Cdk Complexes Shuttle between the ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC99617/
    (His) 6-tagged Cks1 was purified by Ni 2+-affinity chromatography and coupled onto Ultralink biosupport medium (Pierce). HeLa cells transfected with either pcDNA3-myc-cyclin A or with pcDNA3-myc-cyclin A M210A, L214A, W217A M210A were lysed in 0.1% Nonidet P40, 50 mM Tris pH 8.0, 150 mM NaCl, 0.1 mM EDTA and a protease inhibitor cocktail (Roche ...

ISOLATION AND MOLECULAR CLONING OF A SECRETED ...

    https://www.journalofparasitology.org/doi/full/10.1645/0022-3395%282000%29086%5B0516%3AIAMCOA%5D2.0.CO%3B2
    A 36-kDa immunosuppressant protein (Da-p36) was isolated from salivary glands of feeding female ixodid ticks Dermacentor andersoni, using its affinity for UltraLink™ Biosupport Medium (Pierce, Rockford, Illinois)/protein complexes. Using a nested set of forward degenerate oligonucleotide primers corresponding to Da-p36 N-terminal amino acids, a cDNA encoding the immunosuppressant protein …

Capsule Enhances Pneumococcal Colonization by Limiting ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828419/
    To generate a column to assess migration, mucins extracted from human upper airway secretions were covalently linked to acrylamide beads using UltraLink Biosupport according to the manufacturer's instructions (Pierce, Rockford, IL). Binding of mucins was monitored for …

PlexinD1 Glycoprotein Controls Migration of Positively ...

    https://www.sciencedirect.com/science/article/pii/S1074761308004986
    Dec 19, 2008 · (B) PlexinD1-Fc was conjugated to UltraLink Biosupport (Pierce, NJ). Sema3E-Fc and sema3F-Fc were incubated with crosslinked plexinD1-Fc. After washing, proteins were eluted by boiling in protein sample buffer and analyzed by Coomassie brilliant blue staining after resolution on SDS-PAGE.

Pierce ImmunoPure Immobilized Proteins, Thermo Scientific ...

    https://carefordescientific.com/thermo-scientific-pierce-immunopure-immobilized-proteins-20421-cross-linked-6-agarose-7-mg-human-igg-ml-each/
    Click the button below to add the Pierce ImmunoPure Immobilized Proteins, Thermo Scientific # 20421 ( PI20421 ) - Cross-Linked 6% Agarose, 7 mg Human IgG/mL, Each to your wish list.

ImmunoPure IgM Purification Kit from Pierce Biotechnology ...

    http://www.bio-medicine.org/biology-products/ImmunoPure-IgM-Purification-Kit-from-Pierce-Biotechnology--Inc--2786-1/
    ImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.,IgM binds poorly to Protein A and, until recently, no readily available affinity chromatography product existed for one- step IgM purification. Standard methods for IgM purification generally are multi-step, tedious processes, or they are not effective for removing all of the major impurities prese,biological,biology supply ...

Epitope discovery from natural peptide libraries: a new ...

    http://www.biosci.missouri.edu/SmithGP/Miscellaneous%20protocols/UBQfusionsGPSmith.doc
    The FLAG-UBQ fusion protein was diluted to 2 mg/ml in PBS and mixed with an equal volume of coupling buffer (1 M trisodium citrate, 0.2 M NaHCO3, pH adjusted to 8.5 with NaOH); 0.625 gram of amine-reactive UltraLink biosupport medium (Pierce) was suspended in 10 ml of the protein solution, and the suspension was agitated by inversion overnight ...

Mechanisms of autoimmune activation of basophils in ...

    https://www.sciencedirect.com/science/article/pii/S0091674901666120
    The flow-through was also collected. Eluates containing IgG were run through an anti-IgE affinity column to deplete IgE. Immunoaffinity-purified, polyclonal goat anti-human IgE (Chemicon International) was immobilized on Sepharose (UltraLink, Biosupport Medium, Pierce) according to …

Purification - The Wolfson Centre for Applied Structural ...

    https://mafiadoc.com/purification-the-wolfson-centre-for-applied-structural-biology_5ce2609c097c47ae578b466e.html
    Immobilized Protein G Products Pierce Immobilized Protein G Products incorporate the recombinant form of Protein G immobilized to either 6% cross-linked beaded agarose or UltraLink® Biosupport Medium. For a more detailed description of supports, see the previous pages …

Heat shock protein 90 catalyzes activation of the ...

    https://www.pnas.org/content/pnas/99/2/896.full.pdf
    (CTI, Enzyme Research Laboratories) was coupled to Ultralink Biosupport medium (Pierce) according to the manufacturer’s recommendations. In brief, 10 mg of CTI was dissolved in 6 ml of0.1Mcarbonatebuffer(pH9.0),andsodiumcitratewasadded to a final concentration of 0.6 M. To this, 0.25 g of dry beads were added and mixed for2hatroom temperature.



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