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https://en.wikipedia.org/wiki/Hybridization_assay
Overview. In the context of biochemistry and drug development, a hybridization assay is a type of Ligand Binding Assay (LBA) used to quantify nucleic acids in biological matrices. Hybridization assays can be in solution or on a solid support such as 96-well plates or labelled beads.
https://www.ncbi.nlm.nih.gov/pubmed/1703733
Hybridization can be performed either in a liquid solution or on a solid support. In the first case, hybridization is generally followed by a separation step which makes use of solid phase to isolate the hybrid (duplex) formed.Cited by: 2
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hybridization
Hybridization may be carried out in solution or more commonly on a solid-phase support, e.g., nitrocellulose paper. The hybrid of interest is often identified with a radioactively, or alternatively labeled nucleic acid probe or by digestion with an enzyme that specifically attacks single-stranded nucleic acids.
https://pdfs.semanticscholar.org/f82b/5dc0d7dc901f7adb9481f1b2b715e45f2926.pdf
support. Also, only a small amount of the nucleic acid is available for hybridization. These problems can be overcome by using a 'sandwich' hybridization assay. In a sandwich-type assay3-4 the 'target' nucleic acid (analyte) is hybridized to a 'capture' ODN probe that has been covalently immobilized on a solid support.
https://www.sciencedirect.com/science/article/abs/pii/S0003269702000192
Single-nucleotide polymorphism analysis by hybridization protection assay on solid support. ... SNP analysis of the ALDH2 gene or the ApoE gene from human genome samples by solid-phase HPA was successful. Unlike other methods, the microarray by HPA does not require a washing step and can be completed within 30 min. ... Contact and support ...
https://www.researchgate.net/publication/255959569_A_Novel_Method_for_Rapid_Hybridization_of_DNA_to_a_Solid_Support
A Novel Method for Rapid Hybridization of DNA to a Solid Support Article (PDF Available) in PLoS ONE 8(8):e70504 · August 2013 with 63 Reads How we measure 'reads'
https://quizlet.com/88057534/molecular-techniques-in-medicine-flash-cards/
Start studying Molecular techniques in Medicine. Learn vocabulary, terms, and more with flashcards, games, and other study tools. ... - use of hybridization reactions to analyze the nucleic acid content of unknown samples ... Solid-support hybridization
http://www.rsc.org/suppdata/cc/c4/c4cc07153k/c4cc07153k1.pdf
room temperature for 6h. The solid support was then rinsed with 30 mL of dry ACN affording the phosphitylated solid support (4). In order to ensure dry conditions for the subsequent coupling, the solid support 4 was washed with dry ACN (3 times) over an argon atmosphere. To this rinsed solid support (0.5 g), a 0.25M solution of
https://www.ncbi.nlm.nih.gov/pubmed/2278412
As a result a positive hybridization is easily masked by the large excess of unreacted probe molecules, unless a powerful fractionation system is used which removes the free probes. A frequently used method is to immobilize the nucleic acids of the sample on a solid support …Cited by: 4
https://quizlet.com/subject/hybridization-biology/
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