Solid Support Hybridization

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Hybridization assay - Wikipedia

    https://en.wikipedia.org/wiki/Hybridization_assay
    Overview. In the context of biochemistry and drug development, a hybridization assay is a type of Ligand Binding Assay (LBA) used to quantify nucleic acids in biological matrices. Hybridization assays can be in solution or on a solid support such as 96-well plates or labelled beads.

[Solid phase hybridization].

    https://www.ncbi.nlm.nih.gov/pubmed/1703733
    Hybridization can be performed either in a liquid solution or on a solid support. In the first case, hybridization is generally followed by a separation step which makes use of solid phase to isolate the hybrid (duplex) formed.Cited by: 2

Hybridization - an overview ScienceDirect Topics

    https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/hybridization
    Hybridization may be carried out in solution or more commonly on a solid-phase support, e.g., nitrocellulose paper. The hybrid of interest is often identified with a radioactively, or alternatively labeled nucleic acid probe or by digestion with an enzyme that specifically attacks single-stranded nucleic acids.

A versatile solid support system for oligodeoxynucleotide ...

    https://pdfs.semanticscholar.org/f82b/5dc0d7dc901f7adb9481f1b2b715e45f2926.pdf
    support. Also, only a small amount of the nucleic acid is available for hybridization. These problems can be overcome by using a 'sandwich' hybridization assay. In a sandwich-type assay3-4 the 'target' nucleic acid (analyte) is hybridized to a 'capture' ODN probe that has been covalently immobilized on a solid support.

Single-nucleotide polymorphism analysis by hybridization ...

    https://www.sciencedirect.com/science/article/abs/pii/S0003269702000192
    Single-nucleotide polymorphism analysis by hybridization protection assay on solid support. ... SNP analysis of the ALDH2 gene or the ApoE gene from human genome samples by solid-phase HPA was successful. Unlike other methods, the microarray by HPA does not require a washing step and can be completed within 30 min. ... Contact and support ...

A Novel Method for Rapid Hybridization of DNA to a Solid ...

    https://www.researchgate.net/publication/255959569_A_Novel_Method_for_Rapid_Hybridization_of_DNA_to_a_Solid_Support
    A Novel Method for Rapid Hybridization of DNA to a Solid Support Article (PDF Available) in PLoS ONE 8(8):e70504 · August 2013 with 63 Reads How we measure 'reads'

Molecular techniques in Medicine Flashcards Quizlet

    https://quizlet.com/88057534/molecular-techniques-in-medicine-flash-cards/
    Start studying Molecular techniques in Medicine. Learn vocabulary, terms, and more with flashcards, games, and other study tools. ... - use of hybridization reactions to analyze the nucleic acid content of unknown samples ... Solid-support hybridization

synthesis/deprotection/hybridization of RNA An orthogonal ...

    http://www.rsc.org/suppdata/cc/c4/c4cc07153k/c4cc07153k1.pdf
    room temperature for 6h. The solid support was then rinsed with 30 mL of dry ACN affording the phosphitylated solid support (4). In order to ensure dry conditions for the subsequent coupling, the solid support 4 was washed with dry ACN (3 times) over an argon atmosphere. To this rinsed solid support (0.5 g), a 0.25M solution of

DNA hybridization: comparison of liquid and solid phase ...

    https://www.ncbi.nlm.nih.gov/pubmed/2278412
    As a result a positive hybridization is easily masked by the large excess of unreacted probe molecules, unless a powerful fractionation system is used which removes the free probes. A frequently used method is to immobilize the nucleic acids of the sample on a solid support …Cited by: 4

hybridization biology Flashcards and Study Sets Quizlet

    https://quizlet.com/subject/hybridization-biology/
    Learn hybridization biology with free interactive flashcards. Choose from 226 different sets of hybridization biology flashcards on Quizlet.



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