Protein Solid Support

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Chemical Synthesis of Proteins - PubMed Central (PMC)

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2845543/
    The chemical synthesis and semisynthesis of proteins harbor the potential to overcome many of the disadvantages of current protein production methods (19, 29, 78). In particular, chemical synthesis using established solid-phase techniques are rapid to effect, easily automated, and facilitate purification.Cited by: 354

Covalent Attachment of Proteins to Solid Supports and ...

    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001164
    BackgroundThere is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage ...

Covalent Immobilization of Affinity Ligands Thermo ...

    https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/covalent-immobilization-affinity-ligands.html
    Small scale affinity purification using an antibody immobilized to a solid support. Chromatography has three main components: the mobile phase or solvent containing proteins, the stationary or solid phase also called the medium or resin (which may be agarose …

Protein microarray - Wikipedia

    https://en.wikipedia.org/wiki/Protein_array
    A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or ...

Solid-phase synthesis - Wikipedia

    https://en.wikipedia.org/wiki/Solid-phase_synthesis
    In chemistry, solid-phase synthesis is a method in which molecules are covalently bound on a solid support material and synthesised step-by-step in a single reaction vessel utilising selective protecting group chemistry. Benefits compared with normal synthesis in …

Prion Domain of Yeast Ure2 Protein Adopts a Completely ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473064/
    When monomers and aggregates coexist in the sample, it is difficult to distinguish the residual structure in the monomer from the aggregate. To address this problem, we have developed a solid-support EPR approach , in which the protein is tethered on a solid support to prevent protein aggregation. This approach allows the use of high protein ...Cited by: 5



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