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https://www.bdbiosciences.com/us/s/detectionki67
BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care.
https://www.bdbiosciences.com/us/resources/protocols/s/mouseratleukocytes
Immunofluorescent Staining of Mouse and Rat Leukocytes I. Procedure. Harvest cells from tissue, preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cells from murine peripheral blood or a spleen cell suspension can be lysed using BD Biosciences Pharmingen's PharM Lyse™ (Cat. No. 555899) solution.
https://www.bdbiosciences.com/us/resources/protocols/s/brdudetection
SUPPORT PROTOCOLS. Detection of BrdU Incorporation in DNA Synthesizing Cells. NOTE: Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic and carcinogenic. ... BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand ...
https://www.bdbiosciences.com/us/resources/protocols/s/indirectstainingmononuclearcells
SUPPORT PROTOCOLS. Indirect Immunofluorescence Staining of Mononuclear Cells Scope. ... BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. ... Unless otherwise noted, BD, the BD Logo, and all other trademarks are ...
https://www.bdbiosciences.com/us/resources/s/cellsurface
If analysis must be delayed, then the stained cells can be fixed with buffered paraformaldehyde (for example, BD Cytofix buffer, see the product TDS for a detailed protocol) for 30 minutes at 4°C, washed, resuspended in Stain Buffer, and then stored at 4°C (protected from light). The fixed cells should be analyzed as soon as possible.
https://www.bdbiosciences.com/us/resources/protocols/s/paraffinsections
SUPPORT PROTOCOLS. Preparation and Staining of Paraffin Sections. For use with Pharmingen reagents. ... BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care.
https://www.bdbiosciences.com/us/resources/protocols/s/cytokinesfca
Immunofluorescent Staining of Intracellular Cytokines for Flow Cytometric Analysis I. Introduction. Improved methods have continually been sought to analyze single-cell cytokine production by human and rodent leukocytes and cell lines.
http://beta.bdbiosciences.com/services/techsupport/reagents/index.jsp
BD Technical Support Specialists maintain a library of information for customers called the Technical Support Center. It includes technical documentation and protocols for BD Biosciences products and their applications.
http://www.bdbiosciences.ca/eu/resources/s/indirectimmuno
Indirect Immunofluorescence Staining of Human Platelets Procedure for preparation of activated platelets. Centrifuge tube of freshly drawn Sodium Citrate, HEP, or …
https://www.bd.com/en-us/support
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