Affi Prep Protein A Support

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Affi-Prep® Protein A Resin Process Separations Bio-Rad

    https://www.bio-rad.com/en-us/product/affi-prep-protein-resin?ID=8d9b6d24-0bae-463c-aed3-4e70f4d98861
    When Affi-Prep Protein A Resin is used in conjunction with the optimized Affi-Prep Protein A MAPS II Binding Buffer, high-efficiency binding of a wide range of immunoglobulins, including all isotypes of murine IgG, is achieved. Features and Benefits of Affi-Prep Protein A Resin

Affi-Gel® and Affi-Prep® Protein A Resins Life Science ...

    https://www.bio-rad.com/en-us/product/affi-gel-affi-prep-protein-resins?ID=2a45d797-fc0e-4e41-ae99-a80a4bbd1fbf
    With Affi-Gel and Affi-Prep protein A resins and MAPS II buffers, you can purify up to 10 mg of IgG 1 /ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of …

156-0006 156-0005 Catalog Numbers Instruction Manual ...

    http://wolfson.huji.ac.il/purification/PDF/affinity/BIORAD_AffiProtAMatrixInstrMan.pdf
    Affi-Prep Protein A MAPS Preparative Column, 100 x 25 mm 155-0005 Affi-Prep Guard Cartridge, 15 x 25 mm, for all 25 mm ID columns and cartridges 7 Section 3 Ordering Information Catalog Number Product Description 156-0006 Affi-Prep Protein A Support, 5 ml 156-0005 Affi-Prep Protein A Support, 25 ml 153-6164 Affi-Prep Protein A MAPS Buffers ...

Affi-Gel Protein A Support -- 153-6153 - Datasheet Directory

    https://datasheets.globalspec.com/ps/2450/BioRadLaboratories/8ACED2F3-C88B-43EE-AB67-4812373A865B
    5 ml, ready-to-use protein A crosslinked agarose support  Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or …

Affi-Prep Polymyxin Matrix, 25 ml - Hebrew University of ...

    http://wolfson.huji.ac.il/purification/PDF/Endotoxin/BIORAD_AffiPrepPolymyxin.pdf
    Affi-Prep® Polymyxin Matrix, 25 ml Catalog Number 156-0010 Introduction The Affi-Prep polymyxin support is intended for use in low, medium, or high pressure chromatographic applications. The support consists of polymeric macroporous (1,000 Å) 45 µm beads with USP Grade polymyxin B covalently attached. The main application of this

Affi-Prep Protein A Support, 25 ml from Bio-Rad

    http://www.bio-medicine.org/biology-products/Affi-Prep-Protein-A-Support--25-ml-from-Bio-Rad-2748-1/
    Affi-Prep Protein A Support, 25 ml from Bio-Rad,For affinity chromatography, Affi-Prep protein A media yield highly purified immunoglobulins (IgG), selectively remove IgG prior to analysis of other immunoglobulin classes, or adsorb immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from,biological,biology …

Comparison of protein A affinity sorbents - ScienceDirect

    https://www.sciencedirect.com/science/article/pii/S1570023203000928
    Jun 25, 2003 · The boiled sorbent of Protein A Toyopearl exhibits an extreme smear on the gel indicating aggregated and fragmented IgG sticking on the gel after regeneration. Affi-Prep Protein A and Affi-Gel Protein A elute in a very broad peak which is retarded compared to rPrA Sepharose. These sorbents could not be completely regenerated.Cited by: 274

Covalent Coupling of Antibodies to Protein A Beads

    https://www.pottslab.org/protocols/Protein%20Biochemistry/Coupling%20Antibodies.pdf
    Covalent Coupling of Antibodies to Protein A Beads 1. Wash desired amount of Bio-Rad Affi-prep Protein A support (Catalog #153-6153) with 1X PBS three times 2. Add antibody (1 mg antibody per 1 mL solid beads) in 1X PBS (~3-5 mL total) 3. Incubate gently rotating at room temperature for 1 hr 4. Wash beads twice with 0.2 M sodium borate pH 9.0 5.

Affi-Gel Protein A Support from Bio-Rad Biocompare.com

    https://www.biocompare.com/11251-Chromatography-and-Separation-Search-Tool/12089535-Affi-Gel-Protein-A-Support/
    Compare Affi-Gel Protein A Support from Bio-Rad on Biocompare.com

DNA–support coupling for transcription factor purification ...

    https://www.sciencedirect.com/science/article/pii/S0021967301014042
    This result shows that C/EBP, a basic leucine zipper motif protein, also binds by charge (to the control) and by affinity and charge (to the DNA column) and that elution can be quite similar. Download : Download full-size image; Fig. 4. C/EBP binds to the DNA on Affi-Prep support by both charge and affinity.Cited by: 5



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